Mutation in the UL97 open reading frame of human cytomegalovirus strains resistant to ganciclovir
نویسندگان
چکیده
منابع مشابه
Resistance of human cytomegalovirus to cyclopropavir maps to a base pair deletion in the open reading frame of UL97.
Human cytomegalovirus (HCMV) is a widespread pathogen in the human population, affecting many immunologically immature and immunocompromised patients, and can result in severe complications, such as interstitial pneumonia and mental retardation. Current chemotherapies for the treatment of HCMV infections include ganciclovir (GCV), foscarnet, and cidofovir. However, the high incidences of advers...
متن کاملNovel mutation in the UL97 gene of a clinical cytomegalovirus strain conferring resistance to ganciclovir.
Characterization of a ganciclovir-resistant cytomegalovirus strain from a patient with AIDS showed a histidine-to-glutamine change at residue 520 of UL97 (Q520 mutation). In anabolism studies, Q520 was associated with impaired phosphorylation of ganciclovir. Transfer of Q520 to a recombinant virus resulted in a ganciclovir-resistant phenotype.
متن کاملCytomegalovirus UL97 phosphotransferase mutations that affect susceptibility to ganciclovir.
Most ganciclovir (GCV)-resistant cytomegalovirus (CMV) isolates contain UL97 gene mutations at codon 460 or 520 or between codons 590 and 607, where an increasing variety of mutations have been detected, including deletions. To determine their phenotypic effect, 9 UL97 mutations not previously studied were transferred to drug-sensitive laboratory CMV strains that contained unique restriction si...
متن کاملHuman cytomegalovirus UL138 open reading frame is highly conserved in clinical strains.
OBJECTIVE To investigate the variability of human cytomegalovirus (HCMV) UL138 open reading frame (ORF) in clinical strains. METHODS HCMV UL138 ORF was amplified by polymerase chain reaction (PCR) and PCR amplification products were sequenced directly, and the data were analyzed in 19 clinical strains. RESULTS UL138 ORF in all 30 clinical strains was amplified successfully. Compared with th...
متن کاملassessment of the human cytomegalovirus ul97 gene for identification of resistance to ganciclovir in iranian immunosuppressed patients
patients and methods in this study, 87 specimens from iranian patients were amplified using nested pcr amplification of the ul97 gene. sequence analyses of products were performed for identifying the mutated codons. results the present study show that the most frequent gcv-resistant mutations occurred in codons a594v (26.43%), h520q (18.39%), and m460v (13.79%), consequently occurring at a low ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1994
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.68.7.4427-4431.1994